Dephosphorylation of eIF2α-P confirms activation of TORC1 activity in myo1Δwsc1Δ. Western blot analysis of steady state levels of eIF2α and its phosphorylated form eIF2α-P was conducted with 50 μg per lane of whole cell protein extract derived from wt, myo1Δ, wsc1Δ and myo1Δwsc1Δ strains. Pgk1p was used as a loading control. Histograms show the ratio of the relative intensities of each eIF2α band and its Pgk1p loading control, averaged from duplicate experiments. Error bars represent STD Error mean.
Pagán-Mercado et al. BMC Cell Biology 2012 13:13 doi:10.1186/1471-2121-13-13