TORC1 is down regulated and Npr1p dephosphorylation is Sit4p dependent in myo1Δ strains. Protein extracts from A) myo1Δ (40 μg), myo1Δtor1Δ (20 μg) and B) myo1Δsit4Δ (20 μg) mutant strains each expressing an HA-NPR1 plasmid were analyzed by Western blot. Each membrane was probed with anti-HA and anti-PGK1 antibodies. Pgk1p was used as a control. Arrows point to the expected positions of the Npr1pp-phosphorylated form and Npr1p-dephosphorylated form.
Pagán-Mercado et al. BMC Cell Biology 2012 13:13 doi:10.1186/1471-2121-13-13