The TORC1 pathway is down regulated in myo1Δ strains but not in other cell wall stress models. A) Schematic representation of the TOR signaling pathway and regulation of the phosphorylation state of Npr1p following inhibition of TOR by nutrient starvation or rapamycin. B–E) Western blot analysis of HA-NPR1 showing the difference in electrophoretic mobility of phosphorylated Npr1p (Npr1pP) and the dephosphorylated form, Npr1p, following treatments with rapamycin, PPase or a genetic mutation of MYO1 (see Methods for details). Arrows point to the expected positions of the Npr1pp-phosphorylated form (100 kiloDaltons, kDa) and Npr1p-dephosphorylated form (85 kDa). Protein extracts were analyzed from A) wild-type (wt) (10 μg), B) wild-type (wt) (10ug), C) myo1Δ (40ug), and D) chs2Δ (20ug),and E) fks1Δ (20ug).
Pagán-Mercado et al. BMC Cell Biology 2012 13:13 doi:10.1186/1471-2121-13-13