SM22 depletion reduces stress induced ROS production. ROS levels were measured in control REF52 cells (A) or SM22-depleted REF52-depleted cells (B) in normal serum conditions or following serum withdrawal for 24 or 48 h. In control REF52 cells both 24 h or 48 h serum withdrawal resulted in a 15-fold increase in ROS levels. REF52 cells depleted for SM22 (B) however, had 66% lower intrinsic ROS levels, and a considerably reduced ROS response to serum withdrawal for 24 h or 48 h (4-fold and 11-fold increase over untreated respectively). Control cell population is shaded in black, 24 and 48 serum withdrawal populations (24SW and 48SW) are indicated by black or grey lines respectively.
Thompson et al. BMC Cell Biology 2012 13:1 doi:10.1186/1471-2121-13-1