SP cells sorted from Msi1high cells could develop into neural and intestinal epithelial tissues. (A): Proliferative curves of SP and NSP cells cultured in vitro. (B): Developmental potentials of SP and NSP cells in vivo. Abundant AD-like, NT-like, and sack-like structures were observed in the grafts from SP cells. Fibrous tissues and nest-like structures were observed in NSP grafts. Bar indicates 25 μm in this panel. (arrowheads; H&E staining) (C): RT-PCR analysis for the markers of neural cells (Nestin and Tubulin β III) and intestinal epithelium cells (SI, Fabp2, Tff3, Lyz1, and ChgA). (D): Relative mRNA expressions of Nestin and Tubulin β III are shown in this panel. (Mean ± SE; n = 3 individual experiments; *P< 0.05 compared with NSP group; N.D indicates not detected) (E): Relative mRNA expressions of SI, Fabp2, Tff3, Lyz1, and ChgA indicated by the integrated intensity in Panels C. (Mean ± SE; n = 3 individual experiments; *p < 0.05 compared with NSP group; N.D indicates not detected) (F): Immunostaining for Tubulin β III and Fabp2 in SP and NSP grafts. Tubulin β III positive cells were located in some of non-specific structures and neural tube-like structures (arrowheads). More Fabp2 positive cells were detected in SP grafts (arrowheads). Partial Fabp2 positive cells formed sack-like structures with a monolayer cell structure (arrow). The special portion was zoomed and magnified. Bar indicates 50 μm in Tubulin β III panels and 100 μm in Fabp2 panels.
Yu et al. BMC Cell Biology 2011 12:47 doi:10.1186/1471-2121-12-47