Specification of Msi1high cells in vitro derived from mouse ESCs. (A): Relative abundance of mRNA of Msi1 in 5-day EBs (EB, red bar) and induction stage cells at 5-day in EGF medium (EGF, solid bar), SF medium (SF, gray bar), and FCS medium (FCS, blank bar) compared with ESCs (ESC, blue bar). Bars show mean ± SE (n = 3) measured by real-time RT-PCR. The red bar indicates that its abundance is 1.0 on these two scales. (*P< 0.05 compared with all other samples) (B): The immunostaining for Msi1 by immunocytochemistry was detected in partial cells at the 5-day differentiation cultured in EGF, SF, and FCS medium. (arrowheads; Bar indicates 20 μm in this panel) (C): Percentage of Msi1+ cells at the 5-day differentiation cultured in EGF, SF, and FCS medium. (Bar shows mean ± SE in this panel; n = 3 individual experiments; *P< 0.05 compared with all other samples).
Yu et al. BMC Cell Biology 2011 12:47 doi:10.1186/1471-2121-12-47