Cell loss does not accompany serum-withdrawal. HeLa cells were grown for 24 h in MEM/10% NCS and then either left untreated (NT) or washed once with serum-free MEM and then incubated in the same media for 1 h (MEM). A parallel set of samples was treated with water (Water). Cell number was assayed using SYTOX Green and expressed as the percent remaining relative to the untreated sample. Error bars represent the s.d. of the mean (n = 4).
Chirico BMC Cell Biology 2011 12:46 doi:10.1186/1471-2121-12-46