Time course of PGK1 release. After HeLa cells were grown in MEM/10% NBS for 24 h, the cells were washed once with serum-free MEM and then incubated in the same media for the indicated times. The amount of PGK1 and LDH released (A) and remaining in cell lysates (B) was determined and expressed as a percent of the total of each protein in cell lysates at 0 min (untreated cells). Error bars represent the s.d. of the mean (n = 4).
Chirico BMC Cell Biology 2011 12:46 doi:10.1186/1471-2121-12-46