Effect of cell density on PGK1 release. HeLa cells were plated at the indicated cell densities in MEM/10% NBS. After 24 h, the cells were washed with serum-free MEM and then incubated with the same media for 2 h. The amount of PGK1 released into the media was determined using an ELISA. Error bars represent the s.d. of the mean (n = 4). AU, arbitrary units.
Chirico BMC Cell Biology 2011 12:46 doi:10.1186/1471-2121-12-46