Additional file 9.


Movie S6. 'Short' FISH probes detect foci of subnuclear masked transcripts that are distinct from chromatin. FISH against masked SPDS (red) was conducted on 20 μm sections taken from microspores fixed after 30 minutes after hydration. Probes were detected using avidin bound TexasRex. Sections were counterstained with DAPI (blue). Movie is in .mov format playable with QuickTime.

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Boothby and Wolniak BMC Cell Biology 2011 12:45   doi:10.1186/1471-2121-12-45