Additional file 8.
Movie S5. Spermidine additions cause the total dissociation of aggregated nuclear RNA. Microspores were incubated with 10 mM SPD for 4 hours, fixed, sectioned and double stained with DAPI and PY. Subnuclear PY (green) and DAPI (blue) signals were detected in successive confocal slices and these were rendered as a 3D model. Movie is in .mov format playable with QuickTime.
Format: MOV Size: 126KB Download file
Boothby and Wolniak BMC Cell Biology 2011 12:45 doi:10.1186/1471-2121-12-45