Additional file 8.


Movie S5. Spermidine additions cause the total dissociation of aggregated nuclear RNA. Microspores were incubated with 10 mM SPD for 4 hours, fixed, sectioned and double stained with DAPI and PY. Subnuclear PY (green) and DAPI (blue) signals were detected in successive confocal slices and these were rendered as a 3D model. Movie is in .mov format playable with QuickTime.

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Boothby and Wolniak BMC Cell Biology 2011 12:45   doi:10.1186/1471-2121-12-45