Additional file 3.
Movie S1. Pyronin Y labeling reveals discreet aggregations of subnuclear RNA after 2 weeks of dehydration. Microspores collected and fixed after 2 weeks without watering were sectioned and double stained with DAPI and PY. Subnuclear PY (green) signal was detected in successive confocal slices and these were rendered as a 3D model. Movie is in .mov format playable with QuickTime.
Format: MOV Size: 94KB Download file
Boothby and Wolniak BMC Cell Biology 2011 12:45 doi:10.1186/1471-2121-12-45