Additional file 2.

Figure S1. Differential fluorescent labeling of DNA and RNA via dual DAPI/PY staining. Microspores fixed at 30 minutes of development. PY (green) (a, c, e, g, i, k) and DAPI (blue) (b, d, f, h, j, l) detected via 488 nm and UV illumination respectively. a-b, Microspore stained with PY (green). c-d, Microspore stained with DAPI (blue). e-f, Microspore double-stained with DAPI (blue) and PY(green). g-h, Microspore pretreated with RNase and double-stained with both DAPI (blue) and PY (green). i-j, DNase pretreated samples were double-stained with DAPI (blue) and PY (green). k-l, DNase and RNase treated samples were double-stained with DAPI (blue) and PY (green). Bar = 25 μm.

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Boothby and Wolniak BMC Cell Biology 2011 12:45   doi:10.1186/1471-2121-12-45