Table 1

MDCK cell strains sold by three commonly used tissue culture vendors *

Supplier*

Strain

Description

Cat No


ATCC [14]

MDCK (NBL-2)

Parental cell line, heterogeneous population of cells.

CCL-34

Supertube

Isolated from parental line, readily forms tubules in appropriate conditions.

CRL-2285

Superdome

Isolated from parental line, readily forms large domes in appropriate conditions.

CRL-2286

MDCK.1

Isolated from the parental cell line. Used in viral production/studies. Not MDCK type I.

CRL-2935

MDCK.2

Isolated from the parental cell line. Used in viral production/studies. Not MDCK type II.

CRL-2936


ECACC [15]

MDCK (NBL-2)

Parental cell line, same as ATCC cat No. CCL-34.

85011435

MDCK

Parental cell line, heterogeneous population of cells.

84121903

MDCK I

Strain Isolated from a low passage parental cell line.

00062106

MDCK II

Strain isolated from a high passage parental cell line.

00062107

MDCK-Protein free

Strain modified to grow in protein-free medium. Results in growth of suspended clumps of cells.

02050101

MDCK-SIAT1

Parental cell line cloned to give enhanced expression of 6-linked sialic acids. For virus research.

05071502


JCRB [33]

MDCK (NBL-2)

Parental cell line, same as ATCC cat No. CCL-34, unknown passage number.

IFO50071

MDCK (NBL-2)

Parental cell line, same as ATCC cat No. CCL-34, passage number 55-58.

JCRB9029

MDCK.P3

MDCK derivative, no serum required.

JCRB0717


*correct as of September 2011. This article focuses on the ATCC, ECACC and JCRB but other suppliers of cell lines are available, including the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) which does not currently supply MDCK strains

Dukes et al. BMC Cell Biology 2011 12:43   doi:10.1186/1471-2121-12-43

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