Additional file 7.

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Movie 7, Three-dimensional reconstruction of a cell expressing GFP-PTEN (green) and mRFP-LimEΔ (red) as shown in Figure 6B. This reconstruction focuses on the continuity of the PTEN-rich area of the substrate-attached cell membrane with the bulk area of the non-attached membrane. The inner territory circumscribed by the actin wave is depleted of PTEN. The cell has been optically unroofed (white plane).

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Gerisch et al. BMC Cell Biology 2011 12:42   doi:10.1186/1471-2121-12-42