Additional file 4.


Movie 4, Symmetric and asymmetric actin patterns linked to lateral PTEN ingression. The cell co-expresses GFP-PTEN with mRFP-LimEΔ as a label for filamentous actin. The cell is captured in the stage of actin-wave formation during recovery from actin depolymerization using 5 μM latrunculin A. This movie shows the same sequence as Figure 4. Left panel: merged fluorescence images displaying the PTEN label in green and the actin label in red. Middle panel: separate channel showing the fluorescence of GFP-PTEN. Right panel: separate channel showing the fluorescence of mRFP-LimEΔ. Frame-to-frame interval 1 s.

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Gerisch et al. BMC Cell Biology 2011 12:42   doi:10.1186/1471-2121-12-42