Additional file 4.
Movie 4, Symmetric and asymmetric actin patterns linked to lateral PTEN ingression. The cell co-expresses GFP-PTEN with mRFP-LimEΔ as a label for filamentous actin. The cell is captured in the stage of actin-wave formation during recovery from actin depolymerization using 5 μM latrunculin A. This movie shows the same sequence as Figure 4. Left panel: merged fluorescence images displaying the PTEN label in green and the actin label in red. Middle panel: separate channel showing the fluorescence of GFP-PTEN. Right panel: separate channel showing the fluorescence of mRFP-LimEΔ. Frame-to-frame interval 1 s.
Format: MOV Size: 2MB Download file
Gerisch et al. BMC Cell Biology 2011 12:42 doi:10.1186/1471-2121-12-42