Additional file 5.
pAktSer473 phosphorylation affects cellular movement in D562 cells. (A) (B) 5 × 105 YES5 cells (low PPL expression) and D562 cells (high PPL expression) were injected beneath right thigh of 8-week male nude mice and monitored subsequent tumor growth. No tumor growth was observed in YES5 mice, while some tumors were observed in D562 mice. (C) LY294002, a PI3K inhibitor, suppressed pAktSer473 phosphorylation but did not alter PPL expressio in D562 cells. (D) LY294002 suppressed D562 cell migration revealed by wound-healing assay in D562 cells. (E) The ratio of width at 6 hrs/0 hr (%) indicated that LY294002 suppressed wound-healing in D562 cells.
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Tonoike et al. BMC Cell Biology 2011 12:41 doi:10.1186/1471-2121-12-41