Downregulation of PPL by siRNA decreased the number of attached D562 cells. (A) PPL expression was detected in human pharyngeal (D562 and FaDu) or esophageal (YES5, TE2, TE4, TE9, TE11, and T.Tn) cell lines by western blotting. (B) siRNA1 and siRNA2 against PPL was shown in D562 cells. Total cell extracts were collected 72 h after siRNA treatment, and 5 μg protein aliquots were blotted with antibodies against the indicated proteins. siRNA (GL2) against firefly luciferase was used as a control. β-actin was the internal control. (C) Downregulation of PPL inhibited cell growth and decreased the number of attached cells. The number of viable cells was measured at different times by trypan blue exclusion. Values represent the mean ± S.E. from 3 separate experiments. Asterisks (*) indicate a p value less than 0.01 based on a t-test using Day 5 data.
Tonoike et al. BMC Cell Biology 2011 12:41 doi:10.1186/1471-2121-12-41