Isolation, culture and differentiation of rHD/G-DPSCs. (A) Spindle and fibroblast-like cells were observed in rHD/G-DPSC culture (Left), rHD/G-DPSCs express GFP (center) and overlay image (right). (B) Osteogenic differentiation, (C) Adipogenic differentiation, and (D) Chondrogenic differentiation of rHD/G-DPSCs and rG-DPSCs. Osteogenic and adipogenic differentiation were demonstrated by three weeks induction followed by Alizarin Red S and Oil-Red O staining, respectively. Chondrogenic differentiation was demonstrated by four weeks induction followed by Alcian blue staining of cryosection.
Snyder et al. BMC Cell Biology 2011 12:39 doi:10.1186/1471-2121-12-39