Additional file 1.
Testing of rabbit anti-JAG-IC peptide antibody. (A-C) Western Blot after SDS-PAGE with bacterial lysates from E.coli expressing HyJagged-ICD (10 kDa) from pRSET (lanes labelled R JAG-IC 250 ng) and with vesicle fraction from hydra homogenates of 125 animals (lanes labelled V 125 a) probed with anti-JAG-IC antibody (A); Control with rabbit pre-immune serum (B); (C) Control Western blot probed with an unrelated rabbit antibody. This antibody had been raised against a peptide derived from Hydra HES and recognises HyHES (25 kDa) in bacterial lysates from E.coli expressing HyHES from pREST (lane RHyHES 50 ng). (D-G) HEK293T cells expressing HyJagged-GFP from pcDNA3; (D) DNA staining with TO-PRO3, (E) HyJagged-GFP, (F) anti-JAG-IC staining, (G) merged images in false colours: DNA (blue), HyJagged-GFP (green), anti-JAG-IC staining (red); Confocal sections; scale bar: 5 μm.
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Prexl et al. BMC Cell Biology 2011 12:38 doi:10.1186/1471-2121-12-38