Differentiated cells of all protocols were labeled with DIO and seeded on brain slices on day 9. After 10 days coculture slices were propidium iodide stained and scanned with a Leica Microsystems TCS SP2 confocal microscope to measure cell survival and their migration. Arrows indicate several cells that have migrated deeper into the brain tissue. The images were taken at 100× magnification and the brain slices were scanned to a depth of 160 μm.
Hinze and Stolzing BMC Cell Biology 2011 12:35 doi:10.1186/1471-2121-12-35