Figure 5.

Coculture with living brain slices. Differentiated ACM/GM-CSF treated BMC were labeled with DIO and seeded on brain slices on day 9. Counterstaining with propidium iodide was used to assess cell survival. After 1, 2, 3, 6 and 10 days slices were scanned with a Leica Microsystems TCS SP2 confocal microscope to assess survival of seeded cells and their migration into the tissue. Arrows indicate single cells that have already migrated through the surface after 2 days. Magnification was 100x and scanning depth was 160 μm.

Hinze and Stolzing BMC Cell Biology 2011 12:35   doi:10.1186/1471-2121-12-35
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