Figure 3.

Phagocytosis and Oxidative burst of differentiated cells. (A) Phagocytosis of differentiated cells and fluorescence microscope picture of phagocytosis of non adherent BM cells differentiated with ACM/GM-CSF (n = 3). Arrows indicate the same cell in bright field and fluorescence picture. Fluorescence images were taken with a Zeiss Axio Observer at 200× (left) and 400× (right) magnification. (B) Oxidative burst of differentiated cells, representative histogram plot of inactive (open histogram) and PMA activated (red histogram) cells (n = 3). The shift between un-treated and PMA treated microglia was measured as quotient between medians of treated and untreated cells (Fluorescence signal noise ratio - FSN). Cells with no shift are based at 1 and higher numbers represent cell populations which did show ROS production. Light microscope picture of NBT reduction of non adherent BM cells supplemented with ACM/GM-CSF. Arrows indicate one cell with dark blue NBT precipitate and one cell without precipitate. The picture was taken with a Leica DM IL at 20× magnification. *** = P < 0.001, ** = P < 0.01, * = P < 0.05.