Table 1 |
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|
Primers for RT-PCR analysis |
||||
|
Genbank acc no. |
Gene |
5'-Forward primer-3' |
5'-Reverse primer-3' |
Size (bp) |
|
|
||||
|
ChM-I |
CATCGGGGCCTTCTACTTCT |
CTGCGTCGTCCTGAACATTGGG |
584 |
|
|
|
||||
|
Prl8a2 |
TGAATGTCAAACAGGAGAGAA |
CAATCTTGCCCAGTTATGCGG |
628 |
|
|
|
||||
|
Prl3d1 |
GCTTCCATCCATACTCCAG |
TCGTTCTGAAAGACAACTCG |
419 |
|
|
|
||||
|
Brachyury |
CTGCAGTCCCATGATAACTGGTCTAGC |
CCAGGATTTCAAAGTCACATATATGTTGTAG |
717 |
|
|
|
||||
|
Col4a1 |
TGTCCAAGGCAACGAGCGTG |
TGTTCTTCTCATGCACACTTGG |
583 |
|
|
|
||||
|
Col1a2 |
GAACGGTCCACGATTGCATG |
GGCATGTTGCTAGGCACGAAG |
401 |
|
|
|
||||
|
VEGF-A |
CAGAGAGCAACATCACCATGC |
TCCTCGAAGGATCTCCTCTTCC |
313, 445 |
|
|
|
||||
|
Flk-1 |
CACAGACACCACCGTGTACTCC |
CTCCAAGGTAGACAGACTCGGC |
516 |
|
|
|
||||
|
Flt-1 |
TATGGTCTGTGTGCTTAGGTCG |
ACAGGACCATCTATGGTCTTCC |
476 |
|
|
|
||||
|
FGF-2 |
AGCGGCATCACCTCGCTTCC |
TGGAAGCAGTATGGCCTTCTGTCC |
433 |
|
|
|
||||
|
FGFR-1 |
AGAGACCAGCTGTGATGA |
GGCCACTTTGGTCACACG |
461 |
|
|
|
||||
|
IGF-I |
GGACCAGAGACCCTTTGCGGGG |
GGCTGCTTTTGTAGGCTTCAGTGG |
210 |
|
|
|
||||
|
IGF-IR |
ATGCTGTTTGAACTGCAGCGCATGTGCTGG |
CCGCTCGAGCTTGCGGCCCCCGTTCAT |
354 |
|
|
|
||||
|
MMP-3 |
GAAATGCAGAAGTTCCTCGG |
GAGTTCCATAGAGGGACTGA |
878 |
|
|
|
||||
|
MMP-9 |
TGTCATCCAGTTTGGTGTCG |
TAGGGCAGAAGCCATACAGT |
433 |
|
|
|
||||
|
MMP-13 |
TGAAGAGACTGAGCGCTGCG |
ATTCTTCCATGTGGTTCCAGCC |
461 |
|
|
|
||||
|
MMP-14 |
ATCATTGAGGTGGATGAGGAGG |
CAGCCAACCAGGTACACTTGG |
614 |
|
|
|
||||
|
TIMP-1 |
AGTAAGGCCTGTAGCTGTGCC |
ACGAGGACCTGATCCGTCC |
467 |
|
|
|
||||
|
TIMP-2 |
AGCAGTGAGCGAGAAGGAGG |
TCACTTCTCTCGATGCAGGC |
450 |
|
|
|
||||
|
TIMP-3 |
GCTACTGCAGCTGGTACCG |
TTGGACTTCTGCCAACTTCC |
518 |
|
|
|
||||
|
GAPDH |
ACCACAGTCCATGCCATCAC |
TCCACCACCCTGTTGCTGTA |
452 |
|
|
|
||||
|
Miura et al. BMC Cell Biology 2011 12:34 doi:10.1186/1471-2121-12-34 |
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