Additional file 3.
Down-regulation of B23 protein level during senescence is not a result of cell cycle block. (A) NIH3T3 cells were grown either in 10% serum or no serum for 24 hrs. The control or starved cells were stained with propidium iodide to analyze the DNA content by a FACS analysis. (B) The percentage of cells in different phases of the cell cycle with or without serum starvation was analyzed by using ModFit V 3.0 software. (C) NIH3T3 cells were fractionated and pure nuclei from the control and serum starved cells were isolated. The total protein concentration was measured using Bradford-Lowry reagent and equal amounts (10 ug) of nucleoli from the control and serum starved cells were ran on an SDS-PAGE to assess the level of B23 protein during G1 cell cycle block.
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kar et al. BMC Cell Biology 2011 12:33 doi:10.1186/1471-2121-12-33