Additional file 1.
Isolation of nucleolus in NIH3T3 cells. (A) Microscopic analysis of an aliquot of the nuclei and nucleolus during the isolation procedure to assess the integrity of the nuclear and nucleolar membrane (B) Western blotting analysis of the nucleolar, nuclear and cytosolic lysate with a specific antibody towards a nucleolar (anti-fibrillarin), nucleoplasmic (anti-Lamin A/C) or cytoplasmic (anti actin) protein marker to test the purity of the cellular fractions. The protein concentration of the lysates were determined using Bradford-Lowry reagent and 10 ug of lyastes from each fraction were run on a SDS-PAGE.
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kar et al. BMC Cell Biology 2011 12:33 doi:10.1186/1471-2121-12-33