Validation of proteomic result (A) Western blotting analysis used to validate the mass spectrometric result. NIH3T3 cells were vehicle or sodium butyrate treated for 4 days followed by isolation of nucleoli. The protein concentration was ascertained and then 10 ug of total protein was run on an SDS-PAGE to compare the levels of B23, NDH II and PARP1 proteins. Western blotting was performed with anti-B23, anti-NDH II and anti-PARP1 to validate the levels of these proteins following sodium butyrate induced senescence. (B) Indirect Immunofluorescence showing B23 localization in control and sodium butyrate treated (4 days) NIH3T3 cells.
kar et al. BMC Cell Biology 2011 12:33 doi:10.1186/1471-2121-12-33