Table 3

PCR primer pairs used amplify CCSP and β-actin cDNA fragments of BEAS-2B cells

Target

Oligonucleotide sequence

Tm (°C)

bp

CCSP

F: 5'- GAAACTCGCTGTCACCCTCACC-3'

60

211

R: 5'-TTAATGATGCTTTCTCTGG-3'

β-actin

F: 5'-CCTGTACGCCAACACAGTGC-3';

60

234

R: 5'-ATACTCCTGCTTGCTGATCC-3'

F, forward primer; R, reverse primer; Tm, melting temperature.

Shiyu et al. BMC Cell Biology 2011 12:31   doi:10.1186/1471-2121-12-31