Table 2

PCR primer pairs used amplify CCSP, sPLA2, cPLA2 and β-actin cDNA fragments of rat lung

Target

Oligonucleotide sequence

Tm (°C)

bp


β-actin

F: 5'-AGAGGGAAATCGTGCGTGAC-3'

60

195

R: 5'-CCATACCCAGGAAGGAAGGCT-3'

CCSP

F: 5'-CGGACATCTGCCCAGGATTTCT-3'

60

209

R: 5'-ACACAGAGGACTTGTTAGGAT-3'

sPLA2

F: 5'-CCCCAAGGATGCCACAGATT-3'

60

201

R: 5'-TTCCGGGCAAAACATTCAGC-3'

cPLA2

F: 5'-GAAGTTTGCTCATGCCCAGACCT-3'

60

234

R: 5'-TTCATAGAGCGCCTTCATCACACC-3'


F, forward primer; R, reverse primer; Tm, melting temperature.

Shiyu et al. BMC Cell Biology 2011 12:31   doi:10.1186/1471-2121-12-31

Open Data