Generation of induced pluripotent stem cells from mMSCs. (A) Morphology of iPS cells from mMSCs and TTFs (B) Alkaline phosphatase positive clone formation from mMSCs and TTFs. mMSCs and TTFs were plated at 1 × 105 cells/well or 5 × 104 cells/well and were infected with virus/polybrene-containing supernatants, alkaline phosphatase assay was performed 11 days after transduction. (C) Immunofluorescence analysis of pluripotency-associated transcription factors and surface marker in mMSCs-iPS and TTFs-iPS at passage 7. Scale bar = 100 μm.
Fan et al. BMC Cell Biology 2011 12:30 doi:10.1186/1471-2121-12-30