Reduced DNA damage of mMSCs by culture under low (2%) O2. (A) Morphology of primary mMSCs under 20% O2 and 2% O2, respectively. (B) Number of primary mMSCs cultured 20% O2 and 2% O2. Bone marrow cells were plated at a density of 2 x105 cells/cm2 in 100 mm culture dish and cultured for 5 days under 20% O2 and 2% O2, respectively, and cells were counted by hemocytometer. (C) Micronucleus in immunodepleted mMSCs. Arrows indicate micronuclei in binucleated cells. (D) Comparison of spontaneous micronucleus formation in immunodepleted mMSCs cultured under 20% O2 and 2% O2, respectively. Data are expressed as the mean ± SD. *p < 0.05, **p < 0.01; n = 3. Scale bar = 100 μm. Original magnification in C ×400.
Fan et al. BMC Cell Biology 2011 12:30 doi:10.1186/1471-2121-12-30