LEC proliferation during lymphangiogenesis. Cell proliferation was visualised by immunostaining following BrdU incorporation (red) in vitro, in the lymphatic ring assay (A) and in vivo, in whole mounted burned cornea (B). Proliferating LEC at the tip of capillary bud (arrowhead) are detected both in vitro (A) and in vivo (B). LEC are labeled in green with an anti-LYVE-1 antibody (B). Nuclei are counterstained in blue with Dapi (A) or TO-PRO3 (B). The whole mounted samples are observed under fluorescent (A) or confocal (B) microscope. Scale bars in (A): 100 μm and in (B): 40 μm.
Detry et al. BMC Cell Biology 2011 12:29 doi:10.1186/1471-2121-12-29