Table 4

Impact of pretreatment with blebbistatin on the lateral mobility of flotillin-2 cotransfected with flotillin-1 in freshly isolated T-lymphocytes

Transfection

Pretreatment

MF - SDF-1 (n)

MF + SDF-1 (n)


flo2-mCherry/flo-1-EGFP

No pretreatment

0.43 ± 0.05 (11)

0.11 ± 0.03 (13)

flo2-mCherry/flo1-EGFP

Preincubation for 30 min with blebbistatin (100 μM)

0.49 ± 0.06 (9)

0.09 ± 0.02 (10)


FRAP analysis involving local bleaching of mCherry was carried out as described in the legend of Figure 6 with cells preincubated in the absence or presence of SDF-1, and without or with pretreatment with blebbistatin as indicated. The mobile fraction (MF) was calculated for each individual cell and then averaged from a total of n = 9 - 13 cells observed in 3 independent experiments, as indicated (mean ± s.e.m.).

Affentranger et al. BMC Cell Biology 2011 12:28   doi:10.1186/1471-2121-12-28

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