Table 2

Impact of pretreatment with latrunculin A on the lateral mobility of flotillin-2 cotransfected with flotillin-1 in freshly isolated T-lymphocytes

Transfection

Pretreatment

MF - SDF-1 (n)

MF + SDF-1 (n)


flo2-EGFP/flo-1-mCherry

No pretreatment

0.25 ± 0.03 (11)

0.11 ± 0.04 (12)

flo2-EGFP/flo1-mCherry

Preincubation for 30 min with latrunculin A (1 μM)

0.46 ± 0.07 (12)

0.66 ± 0.07 (11)


FRAP analysis involving local bleaching of EGFP was carried out as described in the legend of Figure 6 with cells preincubated in the absence or presence of SDF-1, and without or with pretreatment with latrunculin A as indicated. The mobile fraction (MF) was calculated for each individual cell and then averaged from a total of n = 11 - 12 cells observed in 3 independent experiments, as indicated (mean ± s.e.m.).

Affentranger et al. BMC Cell Biology 2011 12:28   doi:10.1186/1471-2121-12-28

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