Comparison of methods for the quantification of fluorescence associated with nucleoli. Nucleoli were identified with the DAPI image as indicated in the figure. The different filter operations, which were performed to reduce noise and false positives, are listed on top of each panel. (a) Nucleoli were identified with the DAPI image; Detect dark holes and Median filters were applied. (b) Nucleoli were identified with the DAPI image; Detect dark holes and Erode/dilate filters were applied. (c) As shown in the graph, similar results were obtained with both protocols.
Kodiha et al. BMC Cell Biology 2011 12:25 doi:10.1186/1471-2121-12-25