GFP-tagged hsc70 accumulates in nucleoli during the recovery from heat stress. Transiently transfected HeLa cells synthesizing GFP-hsc70 were grown under normal conditions or exposed to a 1-hour heat shock at 45.5°C and then incubated at 37°C for 3 hours. Nucleolar accumulation of GFP-hsc70 was measured with method 1 in controls (n = 51) and cells recovering from heat stress (n = 71). The results for nucleolar fluorescence are shown as means + STDEV; data were normalized to the control, and significant differences were determined with Student's t-test.
Kodiha et al. BMC Cell Biology 2011 12:25 doi:10.1186/1471-2121-12-25