GGA and AP-1 expression in Drosophila. (A) The relative expression of endogenous GGAs and AP-1 in male or female flies was determined in whole fly homogenates (homog.) or by immunoprecipitations (IP). The final elutions were done with either 0.2% SDS (0.2) or more harshly with 2% SDS (2). Note that AP-1 is expressed in similar amounts, but GGA is expressed >3 fold higher in the males. (B) Male or female flies were dissected into head, thorax and abdomen, Western blotted for AP-1 or GGA, and quantitated in (C). Note that GGA is highly expressed in male abdomens. (D) The relative expression of GGA and AP-1 were compared in the testes of 5 flies compared to 5 abdomens. Coomassie gel shows the relative protein loading.
Hirst and Carmichael BMC Cell Biology 2011 12:22 doi:10.1186/1471-2121-12-22