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Open Access Highly Accessed Open Badges Research article

A potential role for the clathrin adaptor GGA in Drosophila spermatogenesis

Jennifer Hirst1* and Jenny Carmichael2

Author Affiliations

1 University of Cambridge, Cambridge Institute for Medical Research, Cambridge CB2 0XY, UK

2 University of Cambridge, Department of Genetics, Cambridge CB2 3EH, UK

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BMC Cell Biology 2011, 12:22  doi:10.1186/1471-2121-12-22

Published: 20 May 2011

Additional files

Additional file 1:

AP-1 and GGA localisation in somatic cells of the seminal vesicle. Testes squashes were triple labelled for either AP-1 or GGA, and the TGN marker GCC88 and Hoechst. Note the coincidence of labelling between AP-1/GGA and GCC88 in large puncta dispersed throughout the cytoplasm. These structures most likely correspond to Golgi mini stacks. Bar, 20 μm

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Additional file 2:

Acroblast formation in GGA RNAi depleted cells. GGA was depleted ubiquitously using a Tubulin-Gal4 driver, and testes squashes from knockdown male flies (A) and control male flies (B) were triple labelled for GGA, the TGN marker GCC88 and Hoechst. Note that the acroblast in the 'onion stage' spermatid stage appears morphologically unaffected where GGA expression is depleted. Bar, 20 μm

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