Additional file 9.
Time-lapse imaging of Fucci2-expressing NMuMG cells during 3 μM etoposide treatment (Additional file 8). NMuMG/Fucci2 cells were grown on a glass-bottom dish and time-lapse imaging was performed using an LCV100. Cells were treated with 3 μM etoposide. Images were acquired every 22.5 m. Playback speed is 15,000 × real time. Total imaging time = 50 h. Only a small fraction of the NMuMG cells were observed to go though multiple endoreplication cycles like the cell featured in this movie.
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Sakaue-Sawano et al. BMC Cell Biology 2011 12:2 doi:10.1186/1471-2121-12-2