Additional file 5.


Time-lapse imaging of Fucci2-expressing NMuMG cells during 1 μM etoposide treatment. NMuMG/Fucci2 cells were grown on a glass-bottom dish and time-lapse imaging was performed using an LCV100. Cells were treated with 1 μM etoposide. Images were acquired every 22.5 m. Playback speed is 7,200 × real time. Total imaging time = 50 h. Most cells were entered M phase and then underwent "nuclear mis-segregation."

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Sakaue-Sawano et al. BMC Cell Biology 2011 12:2   doi:10.1186/1471-2121-12-2