Additional file 2.


Time-lapse imaging of Fucci2-expressing HeLa cells. HeLa/Fucci2 cells were grown on a glass-bottom dish and time-lapse imaging was performed using an LCV100 microscope (Olympus). Images were acquired every 15.5 minutes (m). Playback speed is 14,000 × real time. Total imaging time = 96 hours (h).

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Sakaue-Sawano et al. BMC Cell Biology 2011 12:2   doi:10.1186/1471-2121-12-2