Additional file 1.
Construction of Fucci2. (A)Constructs with concatenated mCherry and mVenus fused to deletion mutants of human Cdt1 and Geminin. cyan box, Cy motif; pink box, D (destruction) box; black box, NLS (nuclear localization signal). (B) Excitation (broken line) and emission (solid line) spectra of mVenus and mCherry. (C) Fucci2 labels individual G1 phase nuclei in red and S/G2/M phase nuclei yellowish green. (D,E) Characterization of HeLa/Fucci2 cells. Cells showing red [mCherry(+)mVenus(-)], orange [mCherry(+)mVenus(+)], and yellowish green [mCherry(-)mVenus(+)] fluorescence were collected (D), and their DNA contents were stained with Hoechst33342 and measured using FACS (E).
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Sakaue-Sawano et al. BMC Cell Biology 2011 12:2 doi:10.1186/1471-2121-12-2