Reduction of gap-junction communication by AGE-BSA, as evaluated using scrape loading assay. A. Bottom images are phase-contrast micrographs of the upper fluorescent images. The concentration of AGE-BSA (in μg/ml) is indicated in the upper left of each paired images. B. Analysis of the area between the bilateral edges of lucifer yellow transfer and the scrape line is shown in the histogram. Bar, 150 μm. Note that significant decrements were seen in cells treated with 250 μg/ml for 24 and 48 hours, and 500 μg/ml for 24 hours, respectively. *, p < 0.05 compared to the untreated cells. h, hours. The inhibition of gap-junction communication at 500 μg/ml of AGE-BSA for 48 hours was not quantified because cells were not well contacted.
Wang et al. BMC Cell Biology 2011 12:19 doi:10.1186/1471-2121-12-19