Reduced expression of endothelial Cx43 gap junctions by AGE-BSA, as evaluated by immunoconfocal microscopy. The levels of Cx43 decreased as the dose of AGE-BSA increased regardless of 24 or 48-hour treatment. Images A-F and G-L were respectively obtained from cells treated with AGE-BSA for 24 and 48 hours. Compared with the control group (A and G), Cx43 gap junctions (red spots) are reduced as the dose of AGE-BSA increases (B-F and H-L). Blue label, nucleus. The concentration of AGE-BSA and the incubation time are indicated in the upper left of each image. h, hours. All images are of the same magnification. Bar, 50 μm.
Wang et al. BMC Cell Biology 2011 12:19 doi:10.1186/1471-2121-12-19