In situ kidney SABG activity in diabetic and non-diabetic mice. SABG staining was carried out for kidney sections, and renal tubule-BGAVs were measured for both control and diabetic (DM) mice at 3 time points (7, 9 and 13 weeks) at pH 4.0 (A) or pH 6.0 (B). DM mice were analyzed by two independent experiments at the 9- and 13-week time points for each pH. Cross-sections are from kidneys at the 13-week time point, for both control and diabetic mice. A. At all three time points SABG staining was apparent and BGAVs for the diabetic mouse was significantly higher than those for the control mouse (7 weeks p < 0.004; 9 weeks p < 0.0009; 13 weeks p < 10E-8). Values at the 9- and 13- week time points for the DM mice represent the mean of two independent experiments (SD = 35.9 and 27, respectively). A black line in both tissue samples demarcates a positively stained tubule. B. No SABG staining was apparent at pH 6.0 for control and diabetic mice and renal tubule-BGAVs were not significantly different between the groups. Values at the 9- and 13- week time points for DM mice represent the mean of two independent experiments (SD = 4.6 and 18.6, respectively). A black line in both tissue samples demarcates a negatively stained tubule.
Shlush et al. BMC Cell Biology 2011 12:16 doi:10.1186/1471-2121-12-16