Table 3

Summary of investigated parameters in normal esophageal epithelial cells and esophageal cancer cell lines.

ESCC

BAC

EPC-hTERT

OE21

Kyse-410

OE33

OE19


Aurora-A


Aurora A gene copies

2.0 ± 0.2

4.2 ± 0.5

9.1 ± 1.2

7.5 ± 0.9

3.9 ± 0.3


Centromere 20 signals

1.9 ± 0.3

4.1 ± 0.7

4.6 ± 0.9

7.2 ± 0.9

2.9 ± 0.4


Aurora-A mRNA

0.4 ± 0.1

0.8 ± 0.1

2.7 ± 0.3

1.3 ± 0.3

1.6 ± 0.1


Aurora-A protein

++

+

+++

+++

+


Aurora-A/phospho T288

+

+

+

(+)

(+)


Aurora-B


Aurora-B gene copies

1.9 ± 0.4

3.0 ± 0.1

3.6 ± 0.8

2.9 ± 0.7

2.0 ± 0.2


Centromere 17 signals

2.0 ± 0.2

3.0 ± 0.1

3.8 ± 0.7

4.9 ± 0.7

3.9 ± 0.4


Aurora A mRNA

0.7 ± 0.0

1.2 ± 0.3

1.5 ± 0.5

0.7 ± 0.4

0.8 ± 0.2


Aurora-B protein

(+)

+++

++++

++

+


Aurora-B/phospho T232

(+)

+

+

+

-


p53


Mutations

no mutation

c.269C>T, c.270delC, p.S90fs31X

c.1009C>T, p.R337C

c.404G>A, p.C135Y

c.928_930insA, p.N310fs26X


p53 protein (kDa)

53

~14 (truncated)

53

53

~40 (truncated)


p53 functionality

+

-

+/-

+

+/-


Mitoses


Mitotic Index (%)

1.0 ± 0.0%

4.6 ± 1.2%

2.2 ± 0.4%

3.4 ± 2.2%

3.2 ± 1.9%


Multipolar mitoses (%)

0.3 ± 0.6%

7.7 ± 5.0%

6.3 ± 2.0%

13.8 ± 4.2%

1.0 ± 1.0%


Quantitative data of each three independent experiments are summarized for FISH and q-RT-PCR (relative mRNA expression) analyses as well as for mitoses and multipolar mitoses (mean and ± standard deviation). Immunoblot analyses are depicted with minus (-) to increasingly positive (+ to ++++) protein expression. Identified p53 mutations and associated effects are listed.

Fichter et al. BMC Cell Biology 2011 12:13   doi:10.1186/1471-2121-12-13

Open Data