Table 5

List of primers used for qPCR (GAPDH is used as a calibrator)

Gene Product

Primers

Product Size (bp)


HoxA11 (NM_005523.5)

Fw: 5'-TTGAGCATGCGGGACAGTT-3'

Rv: 5'-GTACCAGATCCGAGAGCTGGAA-3'

87

OxCT2 (NM_022120.1)

Fw: 5'-GAGTTCAACGGCGACCACTT-3'

Rv:5'-GCGCTTCTCCTGAAGACCA-3'

110

V-KIT (NM_000222.2)

Fw: 5'-GGCGACGAGATTAGGCTGTT-3'

Rv: 5'-CATTCGTTTCATCCAGGATCTCA-3'

77

CCL2 (NM_002982.3)

Fw: 5'-ACTCTCGCCTCCAGCATGAA-3'

Rv: 5'-GGGAATGAAGGTGGCTGCTA-3'

72

CX3CL1 (NM_002996.3)

Fw: 5'-TGACATCAAAGATACCTGTAGC-3'

Rv: 5'-CTCGTCTCCAAGATGATTGC-3'

88

WNT4 (NM_030761.4)

Fw: 5'-AGCAACTGGCTGTACCTG-3'

Rv: 5'-CTGGATCAGGCCCTTGAG-3'

87

GAPDH (NM_002046.3)

Fw: 5'-CGCTCTCTGCTCCTCCTGTT-3'

Rv: 5'-CCATGGTGTCTGAGCGATGT-3'

81


Basciano et al. BMC Cell Biology 2011 12:12   doi:10.1186/1471-2121-12-12

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