Table 4

List of primers for amplification of lineage-specifics transcripts (GAPDH is used as control)

Gene Product

Primers

Product Size (bp)


GAPDH (NM_002046.3)

Fw: 5'-AATCCCATCACCATCTTCCAGG-3'

Rv: 5'-AGAGGCAGGGATGATGTTCTGG-3'

417

ALPL

(NM_000478)

Fw: 5'-CTGGACCTCGTTGACACCTG-3'

Rv: 5'-GCGGTGAACGAGAGAATGTC-3'

546

LPL

(NM_000237.2)

Fw: 5'-AAAGCCCTGCTCGTGCTGAC-3'

Rv: 5'-ACAGGATGTGGCCCGGTTTA-3'

406

PPARG (NM_005037.5)

Fw: 5'-GGAGAAGCTGTTGGCGGAGA-3'

Rv: 5'-CACAATGCTGGCCTCCTTGA-3'

431

RUNX-2 (NM_001015051.2)

Fw: 5'-AACTTCCTGTGCTCGGTGCTG-3'

Rv: 5'-GGGGAGGATTTGTGAAGACGG-3'

268


Basciano et al. BMC Cell Biology 2011 12:12   doi:10.1186/1471-2121-12-12

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