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Open Access Research article

Requirement of Osteopontin in the migration and protection against Taxol-induced apoptosis via the ATX-LPA axis in SGC7901 cells

Rihua Zhang1, Jing Wang1, Shijie Ma1, Zuhu Huang2 and Guoxin Zhang1*

Author Affiliations

1 Department of Gastroenterology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China

2 Department of Infectious Disease, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China

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BMC Cell Biology 2011, 12:11  doi:10.1186/1471-2121-12-11

Published: 16 March 2011

Abstract

Background

Autotaxin (ATX) possesses lysophospholipase D (lyso PLD) activity, which converts lysophosphatidylcholine (LPC) into lysophosphatidic acid (LPA). The ATX-LPA signaling axis has been implicated in angiogenesis, chronic inflammation and tumor progression. Osteopontin (OPN) is an important chemokine involved in the survival, proliferation, migration, invasion and metastasis of gastric cancer cells. The focus of the present study was to investigate the relationship between the ATX-LPA axis and OPN.

Results

In comparison with non-treated cells, we found that the ATX-LPA axis up-regulated OPN expression by 1.92-fold in protein levels and 1.3-fold in mRNA levels. The ATX-LPA axis activates LPA2, Akt, ERK and ELK-1 and also protects SGC7901 cells from apoptosis induced by Taxol treatment.

Conclusions

This study provides the first evidence that expression of OPN induced by ATX-LPA axis is mediated by the activation of Akt and MAPK/ERK pathways through the LPA2 receptor. In addition, OPN is required for the protective effects of ATX-LPA against Taxol-induced apoptosis and ATX-LPA-induced migration of SGC7901 cells.