Table 2

Role of ROS in CO prevention of inner membrane permeabilization

Control

Ca2+ 5 μM

CO 10 μM

CO 10 μM Ca2+ 5 μM

CO 10 μM Ca2+ 5 μM ß-c 1 μM


Slope (Abs/min)

0,00E + 00

1,78E - 02

00E00

9,60E - 03

1,34E - 02

% ΔSlope (Abs/min) relative to Ca2+

0,00

100,00 ± 0,68

0,00

53,79 ± 0,56

75,22 ± 0,65


Inner membrane permeabilization is followed by absorbance at 412 nm. The slope was calculated between 10 to 20 minutes for each different condition in order to assess the effect of CO in the inner membrane permeabilization in the presence or absence of ß-carotene and after treatment with Ca2+. For slope calculation, absorbance values for each different condition were normalized by the absorbance value corresponding to the control non-treated mitochondria. The difference between control mitochondria and Ca2+ -treated mitochondria slopes correspond to an increase of 100%.

Queiroga et al. BMC Cell Biology 2011 12:10   doi:10.1186/1471-2121-12-10

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